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01/12/2010
Lucid Fall's interview in Apr 2001
http://www.personweb.com/viewInterview.jsp?mm=I&idx=59
Jan 12, 2010 10:01:37 AM
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Engineering integrin signaling for promoting embryonic stem cell self-renewal in a precisely defined niche.
Lee ST from Hubbell lab, Biomaterials 09 (Yunsuk Jo as a mid author) We present development and use of a 3D synthetic extracellular matrix (ECM) analog with integrin-specific adhesion ligands to characterize the microenvironmental influences in embryonic stem cell (ESC) self-renewal. Transcriptional analysis of 24 integrin subunits followed by confirmation at the translational and functional levels suggested that integrins alpha(5)beta(1), alpha(v)beta(5), alpha(6)beta(1) and alpha(9)beta(1) play important roles in maintenance of stemness in undifferentiated mouse ESCs. Using the well-defined matrix as a tool to activate integrins alpha(5)beta(1) plus alpha(v)beta(5), alpha(6)beta(1) and alpha(9)beta(1), individually and in combination, differential integrin activation was demonstrated...
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